Development and Validation of a Two-step TaqMan Real-time RT-PCR for the Detection of Infectious Bursal Disease Virus in Infected Chickens
Fatima Tahiri, Mohammed Bourhia, EL Harrak Mehdi, Benaissa Attarassi, Riaz Ullah, Hafiz Majid Mahmood, Ahmed bari, Laila Benbacer and Driss Belghyti
Abstract:
Keywords: IBDV; Tow-step TaqMan rRT-PCR; SYBR Green -RT-PCR; Diagnosis; Assay validation
Edition: Volume 24 Issue 06, 2020
Pages: 1773-1780
1 Division of Pharmacy and Veterinary Inputs, National Laboratory of Veterinary Drug Control, ONSSA, PB 4590 Rabat, Morocco
2 Laboratory of Chemistry-Biochemistry, Environment, Nutrition, and Health, Faculty of Medicine and Pharmacy, Hassan II University B.P 5696, Casablanca, Morocco
3 Society of Biological Production and Veterinary Pharmaceutical Products, Biopharma Akkari quarter, km2 Casa road - PB 4569 10000 Rabat, Morocco
4 Laboratory of Biology and Health, Faculty of Sciences, IbnTofail University, 14000 Kenitra, Morocco
5 Department of Pharmacognosy, College of Pharmacy, King Saud University, P. O. Box 2457, Riyadh 11451, Saudi Arabia
6 Department of Pharmacology, College of Pharmacy, King Saud University, P. O. Box 2457, Riyadh 11451, Saudi Arabia
7 Department of Pharmaceutical Chemistry, College of Phamacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia
8 Research Unit and Medical Biology, National Center for Nuclear Energy, Science, and Technology (CNESTEN), Rabat 10001, Morocco
For correspondence: bourhiamohammed@gmail.com; tahirifatima.dpiv@gmail.com; rullah@ksu.edu.sa
Received 07 May 2020; Accepted 04 August 2020; Published 10 October 2020
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