Abstract

Coenzyme Q10 (CoQ10), a potential of antioxidant, has been hypothesized to mitigate oxidative stress and improve sperm quality after freezing. By understanding the impact of CoQ10 in the LREY diluent, the study aims to provide insights into potential improvements in cryopreservation techniques, which are crucial for poultry breeding and genetic conservation efforts. The study aims to determine the CoQ10 in the Lactate ringer egg yolk (LREY) diluent on the quality of the chicken's sperm after freezing. The samples were divided into four different treatment groups, namely, containing samples of semen + LREY (P0), semen + LREY+ CoQ10 100 μM (P1), semen + LREY+ CoQ10 200 μM (P2) and (P3) semen + LREY+ CoQ10 300 μM. The variables include sperm motility, sperm viability, sperm abnormality, sperm plasma membrane integrity and sperm DNA damage. The data were analyzed using the one-way ANOVA and continued with the Duncan test. The results showed no significant influence on the treatment of P0, P1, P2 and P3 on sperm motility, sperm viability, sperm abnormality, sperm plasma membrane integrity of gaga chicken semen during freezing (P > 0.01). However, there was a significant effect on sperm DNA damage (P < 0.01). The results are expected to contribute to the development of more effective sperm preservation methods, thereby supporting the poultry industry's productivity and genetic diversity.